mcf10a wildtype cells Search Results


wildtype mcf10a cells  (ATCC)
99
ATCC wildtype mcf10a cells
(A) Background-corrected FRET ratio traces for <t>MCF10A</t> cells expressing NLS-ZapCV2. Addition of ZnCl 2 for 30 min results in an increase in labile Zn 2+ from 150 pM to 75 nM. Addition of the Zn 2+ chelator TPA followed by the addition of Zn 2+ and pyrithione at 75 min allows for calibration of the sensor and subsequent quantification of labile Zn 2+ . (B) Quantification of Zn 2+ at rest and at the time point of ATAC-seq. (C) Pseudo-colored ratio images of representative cells at the indicated points. (D) FRET ratio traces of MCF10A cells treated with TPA to deplete labile Zn 2+ , followed by calibration. (E) Quantification of Zn 2+ at rest. The asterisk indicates that Zn 2+ cannot be accurately quantified because it is at the lower detection limit of the sensor. Zn 2+ is estimated to be ∼1 pM. (F) Pseudo-colored ratio images of representative cells at the indicated points. Each trace represents a single cell in the field of view. Lookup table values refer to the FRET ratio (background-corrected FRET channel/background-corrected CFP channel). Scale bar = 20 μm.
Wildtype Mcf10a Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wildtype mcf10a cells/product/ATCC
Average 99 stars, based on 1 article reviews
wildtype mcf10a cells - by Bioz Stars, 2026-05
99/100 stars
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brca1 antibody (17f8)  (Bio-Techne corporation)
94
Bio-Techne corporation brca1 antibody (17f8)
(A) Background-corrected FRET ratio traces for <t>MCF10A</t> cells expressing NLS-ZapCV2. Addition of ZnCl 2 for 30 min results in an increase in labile Zn 2+ from 150 pM to 75 nM. Addition of the Zn 2+ chelator TPA followed by the addition of Zn 2+ and pyrithione at 75 min allows for calibration of the sensor and subsequent quantification of labile Zn 2+ . (B) Quantification of Zn 2+ at rest and at the time point of ATAC-seq. (C) Pseudo-colored ratio images of representative cells at the indicated points. (D) FRET ratio traces of MCF10A cells treated with TPA to deplete labile Zn 2+ , followed by calibration. (E) Quantification of Zn 2+ at rest. The asterisk indicates that Zn 2+ cannot be accurately quantified because it is at the lower detection limit of the sensor. Zn 2+ is estimated to be ∼1 pM. (F) Pseudo-colored ratio images of representative cells at the indicated points. Each trace represents a single cell in the field of view. Lookup table values refer to the FRET ratio (background-corrected FRET channel/background-corrected CFP channel). Scale bar = 20 μm.
Brca1 Antibody (17f8), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brca1 antibody (17f8)/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
brca1 antibody (17f8) - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier

Image Search Results


(A) Background-corrected FRET ratio traces for MCF10A cells expressing NLS-ZapCV2. Addition of ZnCl 2 for 30 min results in an increase in labile Zn 2+ from 150 pM to 75 nM. Addition of the Zn 2+ chelator TPA followed by the addition of Zn 2+ and pyrithione at 75 min allows for calibration of the sensor and subsequent quantification of labile Zn 2+ . (B) Quantification of Zn 2+ at rest and at the time point of ATAC-seq. (C) Pseudo-colored ratio images of representative cells at the indicated points. (D) FRET ratio traces of MCF10A cells treated with TPA to deplete labile Zn 2+ , followed by calibration. (E) Quantification of Zn 2+ at rest. The asterisk indicates that Zn 2+ cannot be accurately quantified because it is at the lower detection limit of the sensor. Zn 2+ is estimated to be ∼1 pM. (F) Pseudo-colored ratio images of representative cells at the indicated points. Each trace represents a single cell in the field of view. Lookup table values refer to the FRET ratio (background-corrected FRET channel/background-corrected CFP channel). Scale bar = 20 μm.

Journal: Life Science Alliance

Article Title: Cellular zinc status alters chromatin accessibility and binding of p53 to DNA

doi: 10.26508/lsa.202402638

Figure Lengend Snippet: (A) Background-corrected FRET ratio traces for MCF10A cells expressing NLS-ZapCV2. Addition of ZnCl 2 for 30 min results in an increase in labile Zn 2+ from 150 pM to 75 nM. Addition of the Zn 2+ chelator TPA followed by the addition of Zn 2+ and pyrithione at 75 min allows for calibration of the sensor and subsequent quantification of labile Zn 2+ . (B) Quantification of Zn 2+ at rest and at the time point of ATAC-seq. (C) Pseudo-colored ratio images of representative cells at the indicated points. (D) FRET ratio traces of MCF10A cells treated with TPA to deplete labile Zn 2+ , followed by calibration. (E) Quantification of Zn 2+ at rest. The asterisk indicates that Zn 2+ cannot be accurately quantified because it is at the lower detection limit of the sensor. Zn 2+ is estimated to be ∼1 pM. (F) Pseudo-colored ratio images of representative cells at the indicated points. Each trace represents a single cell in the field of view. Lookup table values refer to the FRET ratio (background-corrected FRET channel/background-corrected CFP channel). Scale bar = 20 μm.

Article Snippet: Wildtype MCF10A cells (#CRL-10317; ATCC) were cultured at 5% CO 2 in DMEM/F12 (#11320033; Thermo Fisher Scientific) supplemented with 5% Horse Serum (#16050122; Thermo Fisher Scientific), 1% penicillin/streptomycin (#15070063; Thermo Fisher Scientific), 20 ng/ml EGF (#PHG0313; Thermo Fisher Scientific), 0.5 mg/ml hydrocortisone (#H0888; Sigma-Aldrich), 100 ng/ml Cholera toxin (#C8052; Sigma-Aldrich, and 10 μg/ml insulin (#12585014; Thermo Fisher Scientific).

Techniques: Expressing

(A) Barcode plots that show enrichment of the p 53 motif upon treatment of MCF10A cells with TPA (top). Addition of exogenous ZnCl 2 results in depletion of the same motif (bottom). The barcode plots represent each quartile (Q1–Q4) of the enrichment curves generated for the p53 motif. Red indicates more enrichment of the motif; yellow is less enrichment of the motif. (B) A subset of p53 binding sites from a ChIP-seq dataset ( GSM3378513 ) are differentially accessible depending on cellular Zn 2+ status. ATAC-seq reads were mapped to 2,164 ChIP-seq peaks and subjected to differential accessibility analysis using DESeq2. With TPA treatment, 62 peaks showed significant ( P adj ≤ 0.1) changes in accessibility (51 decreased, 11 increased). With ZnCl 2 treatment, seven peaks showed significant changes in accessibility (one increased, five decreased). Source data are available for this figure.

Journal: Life Science Alliance

Article Title: Cellular zinc status alters chromatin accessibility and binding of p53 to DNA

doi: 10.26508/lsa.202402638

Figure Lengend Snippet: (A) Barcode plots that show enrichment of the p 53 motif upon treatment of MCF10A cells with TPA (top). Addition of exogenous ZnCl 2 results in depletion of the same motif (bottom). The barcode plots represent each quartile (Q1–Q4) of the enrichment curves generated for the p53 motif. Red indicates more enrichment of the motif; yellow is less enrichment of the motif. (B) A subset of p53 binding sites from a ChIP-seq dataset ( GSM3378513 ) are differentially accessible depending on cellular Zn 2+ status. ATAC-seq reads were mapped to 2,164 ChIP-seq peaks and subjected to differential accessibility analysis using DESeq2. With TPA treatment, 62 peaks showed significant ( P adj ≤ 0.1) changes in accessibility (51 decreased, 11 increased). With ZnCl 2 treatment, seven peaks showed significant changes in accessibility (one increased, five decreased). Source data are available for this figure.

Article Snippet: Wildtype MCF10A cells (#CRL-10317; ATCC) were cultured at 5% CO 2 in DMEM/F12 (#11320033; Thermo Fisher Scientific) supplemented with 5% Horse Serum (#16050122; Thermo Fisher Scientific), 1% penicillin/streptomycin (#15070063; Thermo Fisher Scientific), 20 ng/ml EGF (#PHG0313; Thermo Fisher Scientific), 0.5 mg/ml hydrocortisone (#H0888; Sigma-Aldrich), 100 ng/ml Cholera toxin (#C8052; Sigma-Aldrich, and 10 μg/ml insulin (#12585014; Thermo Fisher Scientific).

Techniques: Generated, Binding Assay, ChIP-sequencing

Also shown are the PRO-seq coverage tracks for MCF10A cells treated with 10 μM Nutlin-3A for 3 h ( GSE227931 ), the annotated region from the GSM3378513 p53 Nutlin-3A ChIP-seq dataset, and the predicted amplicon from ChIP-qPCR. (A, B, C, D, E, F) Coverage tracks as noted above for the (A) ERGIC1, (B) NFIB, (C) SFN, (D) EGR1, (E) PLD5, and (F) LRIG3-DT regions.

Journal: Life Science Alliance

Article Title: Cellular zinc status alters chromatin accessibility and binding of p53 to DNA

doi: 10.26508/lsa.202402638

Figure Lengend Snippet: Also shown are the PRO-seq coverage tracks for MCF10A cells treated with 10 μM Nutlin-3A for 3 h ( GSE227931 ), the annotated region from the GSM3378513 p53 Nutlin-3A ChIP-seq dataset, and the predicted amplicon from ChIP-qPCR. (A, B, C, D, E, F) Coverage tracks as noted above for the (A) ERGIC1, (B) NFIB, (C) SFN, (D) EGR1, (E) PLD5, and (F) LRIG3-DT regions.

Article Snippet: Wildtype MCF10A cells (#CRL-10317; ATCC) were cultured at 5% CO 2 in DMEM/F12 (#11320033; Thermo Fisher Scientific) supplemented with 5% Horse Serum (#16050122; Thermo Fisher Scientific), 1% penicillin/streptomycin (#15070063; Thermo Fisher Scientific), 20 ng/ml EGF (#PHG0313; Thermo Fisher Scientific), 0.5 mg/ml hydrocortisone (#H0888; Sigma-Aldrich), 100 ng/ml Cholera toxin (#C8052; Sigma-Aldrich, and 10 μg/ml insulin (#12585014; Thermo Fisher Scientific).

Techniques: ChIP-sequencing, Amplification, ChIP-qPCR